. . . . "config.yml" . "samples: samples.csv\n\nref:\nspecies: \"Drosophila melanogaster\"\ngenome: \"\"\nannotation: \"\"\naccession: \"GCF_000001215.4\"\nensembl_species: \"\" # e.g., \"homo_sapiens\"\nbuild: \"\" # e.g., \"GRCh38\"\nrelease: \"\" # e.g., \"105\"\n\nread_filter:\nmin_length: 200\n\nminimap2:\nindex_opts: \"\"\nopts: \"\"\nmaximum_secondary: 100\nsecondary_score_ratio: 1.0\n\nsamtools:\nsamtobam_opts: \"-b\"\nbamsort_opts: \"\"\nbamindex_opts: \"\"\nbamstats_opts: \"\"\n\nquant:\nsalmon_libtype: \"U\"\n\ndeseq2:\nfit_type: \"\"\ndesign_factors:\n- \"condition\"\nlfc_null: 1.0\nalt_hypothesis: \"greaterAbs\"\npoint_width: 20\nmincount: 10\nalpha: 0.05\nthreshold_plot: 10\ncolormap: \"Blues\"\nfigtype: \"png\"\nbatch_effect:\n- \"\"\n\nisoform_analysis:\nFLAIR: true\nqscore: 1\nexp_thresh: 10\ncol_opts: \"--annotation_reliant generate --generate_map --stringent\"\n\nprotein_annotation:\nlambda: false\nuniref: \"https://ftp.imp.fu-berlin.de/pub/lambda/index/lambda3/gen_0/uniref50_20230713.lba.gz\"\nnum_matches: 3" . . "RAjHDlPDghZzc9ZvQ3uJQNJ9Jd_KAYzZt7dk5PXKgjRyE" . "This workflow performs differential expression analysis of RNA-seq data obtained from Oxford Nanopore long-read sequencing technology.\nFirst a transcriptome FASTA is constructed using gffread. Reads are then mapped to the transcriptome with the long-read optimized alignment tool minimap2.\nNext quantification is performed using salmon before normalization and differential expression analysis are conducted by PyDESeq2.\nThe workflow can optionally analyze splice-isoforms through integrating the FLAIR workflow.\nAdditionaly, NanoPlot is employed to analyze initial sequencing data and QualiMap is used to evaluate mapping results." . "2026-04-16T13:13:39.282684+00:00"^^ . . "from workflow configuration" . . "2026-04-16T15:13:43.874001"^^ . . "RSA" . "MIIBIjANBgkqhkiG9w0BAQEFAAOCAQ8AMIIBCgKCAQEAkOFUnnRCp/k9/0ugvx8zQJ+Qc675W1Ug6F839+xvJ2QsSBu4iOJ1O2kJCmb3tALp3gJOt8sffRot3VrfY1hbgXxL7BVtDsfHHmXVff4YCeg5Ycdn5cDpLawDpAdYwMhK0LwIkZ3fwH9/o9JniYKXLV/jpF9bMKyiw/6tqlCHaMW1r8gzZzoxVIAakwvlABoY0iNoToLTlBRXEI4mLUNjDMnMwQgfh1KXMxMruNjW3wJyeDEIfa2ooAt0E4CRM9pkrEb37NzD9Jz8aSUFFY6BvIxF4ixK7rm6IUDvQ76LqXkEmgSeRv1kw7gnCe9wV/wHd0ZeW4heoBXmLHX3MvHfjwIDAQAB" . "R8J92Gbnag+hwaAmGDIa55n00LXiNHGk2lJfPbiafKqm1UnFM5U2eJ8RqZgKwagPPebO+i1Oph8MapTl8SOgEqUTiCKYB496c251LSmqYjnOm6+OhbZE65EcFyrT952WwFSyypiHK1O845nfppyBgzi8Lr+9S2nHUrITZbGdYGhWOsPH6xLNxspIsnpTBmrJob+R/NiyzUf2xvvO++Key1SsmsVrN5/utKF48c7qRLZovpLI7RxezSRCvxAeynfhRKEItygfrc8WTDR0Xn/L5pqJ0+P748U7QHN6uaCR17fKtLsHRi31p0/7s2XyMCMopsyBi+4cX0dWgrkY0xETUA==" . . "2026-04-16T13:13:39.282684+00:00"^^ . . . "Snakemake workflow metadata: RAjHDlPDghZzc9ZvQ3uJQNJ9Jd_KAYzZt7dk5PXKgjRyE" . "2026-04-16T15:13:43.874001"^^ . .